Slc16a1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comSlc16a1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.com

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Slc16a1 (Gene name),

Monocarboxylate transporter 1 (Protein name ), MOT1_RAT from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Slc16a1(Mct1);

Protein name:

Monocarboxylate transporter 1(MCT 1);

Alternative:

Solute carrier family 16 member 1;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.

Subcellular Location:

Cell membrane Multi-pass membrane protein

Protein Attributes:

Sequence length:

494

Sequence:

50:

MPPAIGGPVG | YTPPDGGWGW | AVVVGAFISI | GFSYAFPKSI | TVFFKEIEII |

100:

FSATTSEVSW | ISSIMLAVMY | AGGPISSILV | NKYGSRPVMI | AGGCLSGCGL |

150:

IAASFCNTVQ | ELYFCIGVIG | GLGLAFNLNP | ALTMIGKYFY | KKRPLANGLA |

200:

MAGSPVFLST | LAPLNQAFFG | IFGWRGSFLI | LGGLLLNCCV | AGSLMRPIGP |

250:

QQGKVEKLKS | KESLQEAGKS | DANTDLIGGS | PKGEKLSVFQ | TVNKFLDLSL |

300:

FTHRGFLLYL | SGNVVMFFGL | FTPLVFLSNY | GKSKHFSSEK | SAFLLSILAF |

350:

VDMVARPSMG | LAANTRWIRP | RVQYFFAASV | VANGVCHLLA | PLSTTYVGFC |

400:

IYAGVFGFAF | GWLSSVLFET | LMDLVGPQRF | SSAVGLVTIV | ECCPVLLGPP |

450:

LLGRLNDMYG | DYKYTYWACG | VILIIAGLYL | FIGMGINYRL | VAKEQKAEEK |

494:

KRDGKEDETS | TDVDEKPKKT | MKETQSPAPL | QNSSGDPAEE | ESPV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

10116.ENSRNOP00000027234

Pfam:

UniGene:

String:

KEGG:

Uniprot:

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ELISA Kit for Rat MCT 1

Cat.:

E7726m

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Manual:

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Packing:

96T

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ELISA Kit for Rat MCT 1

Cat.:

E7726r

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Packing:

96T

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ELISA Kit for Rat MCT 1

Cat.:

E7726h

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Packing:

96T

ELISA Kit for Rat MCT 1

Cat.:

E7726b

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MSDS:

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Packing:

96T

CLIA Kit for Rat MCT 1

Cat.:

U7726r

Price:

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Packing:

96T

CLIA Kit for Rat MCT 1

Cat.:

U7726b

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MSDS:

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Packing:

96T

CLIA Kit for Rat MCT 1

Cat.:

U7726h

Price:

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MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Rat MCT 1

Cat.:

U7726m

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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Polyclonal Antibody for Rat MCT 1

Polyclonal Antibody for Rat MCT 1

Cat.:

P7726Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat MCT 1

Cat.:

P7726Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat MCT 1

Cat.:

P7726Rb-h

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Packing:

40ug/0.2ml

Monoclonal Antibody for Rat MCT 1

Monoclonal Antibody for Rat MCT 1

Monoclonal Antibody for Rat MCT 1

Monoclonal Antibody for Rat MCT 1

Protein for Rat MCT 1

Protein for Rat MCT 1

Protein for Rat MCT 1

Protein for Rat MCT 1

R&D Technical Data

s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

s

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.7%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

Recovery was determined by spiking various levels of MOT1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	95-108
Plasma	100	93-105

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MOT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	97-105%	95-109%	93-110%	96-107%
EDTA plasma(n=5)	91-110%	93-108%	92-109%	90-109%
heparin plasma(n=5)	93-106%	94-106%	95-103%	96-104%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"cDNA cloning and functional characterization of rat intestinal monocarboxylate transporter."
Takanaga H. , Tamai I. , Inaba S. , Sai Y. , Higashida H. , Yamamoto H. , Tsuji A.
Biochem. Biophys. Res. Commun.217:370-377(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;TISSUE SPECIFICITY;SUBCELLULAR LOCATION
strain: Wistar.
tissue: Small intestine.
2.

"cDNA cloning of MCT1, a monocarboxylate transporter from rat skeletal muscle."
Jackson V.N. , Price N.T. , Halestrap A.P.
Biochim. Biophys. Acta1238:193-196(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Wistar.
tissue: Skeletal muscle.
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Lung.
4.

"A monocarboxylate transporter MCT1 is located at the basolateral pole of rat jejunum."
Orsenigo M.N. , Tosco M. , Bazzini C. , Laforenza U. , Faelli A.
Exp. Physiol.84:1033-1042(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 264-472;FUNCTION;SUBCELLULAR LOCATION;TISSUE SPECIFICITY
tissue: Jejunal epithelium.
5.

"Interaction of the erythrocyte lactate transporter (monocarboxylate transporter 1) with an integral 70-kDa membrane glycoprotein of the immunoglobulin superfamily."
Poole R.C. , Halestrap A.P.
J. Biol. Chem.272:14624-14628(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH EMB;TISSUE SPECIFICITY;SUBCELLULAR LOCATION
6.

"Fluorescence resonance energy transfer studies on the interaction between the lactate transporter MCT1 and CD147 provide information on the topology and stoichiometry of the complex in situ."
Wilson M.C. , Meredith D. , Halestrap A.P.
J. Biol. Chem.277:3666-3672(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH BSG;FUNCTION;SUBCELLULAR LOCATION
7.

"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
Moser K. , White F.M.
J. Proteome Res.5:98-104(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
8.

"The role of charged residues in the transmembrane helices of monocarboxylate transporter 1 and its ancillary protein basigin in determining plasma membrane expression and catalytic activity."
Manoharan C. , Wilson M.C. , Sessions R.B. , Halestrap A.P.
Mol. Membr. Biol.23:486-498(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH BSG;TOPOLOGY;3D-STRUCTURE MODELING;MUTAGENESIS OF ARG-86; ARG-196; ASP-302 AND ARG-306
9.

"Studies on the DIDS-binding site of monocarboxylate transporter 1 suggest a homology model of the open conformation and a plausible translocation cycle."
Wilson M.C. , Meredith D. , Bunnun C. , Sessions R.B. , Halestrap A.P.
J. Biol. Chem.284:20011-20021(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH EMB;SUBCELLULAR LOCATION;TOPOLOGY;3D-STRUCTURE MODELING;MUTAGENESIS OF LYS-38; LYS-45; LYS-282; LYS-284; LYS-290 AND LYS-413;ENZYME REGULATION;TISSUE SPECIFICITY

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